《植物生理学报》 2015, 51(12): 2181-2187

淹水胁迫对不结球白菜抗坏血酸-谷胱甘肽循环的影响

郭欣欣^1,2, 李晓锋¹, 朱红芳¹, 朱玉英^1,*, 刘金平^1,2

¹上海市农业科学院园艺研究所, 上海市设施园艺技术重点实验室, 上海201106; ²南京农业大学园艺学院, 南京210095

通信作者：朱玉英；E-mail: yy5@saas.sh.cn；Tel: 021-52235455

摘　要：

采用双套盆法模拟淹水实践, 探讨不同涝渍时间及深度(根淹、半淹)处理下不结球白菜幼苗根系中活性氧物质含量及抗坏血酸-谷胱甘肽循环的变化规律。结果表明: 淹水胁迫显著增加了不结球白菜幼苗根系中的H₂O₂含量及O₂ ¯•产生速率, 随着胁迫时间的延长, 半淹处理的升幅更大。胁迫解除后仍未恢复至对照水平。淹水胁迫期间, 不结球白菜幼苗根系中抗氧化物质含量(AsA和GSH)和氧化还原力(AsA/DHA、GSH/GSSG)降低, DHA和GSSG含量则显著升高, 且半淹处理的增幅或降幅均高于根淹处理, 即随胁迫加剧, 不结球白菜所受到的伤害越大。胁迫初期不结球白菜幼苗根系可通过保持相对较高的APX、DHAR和GR活性, 抵御胁迫带来的损伤。综上表明, 不结球白菜幼苗根系在胁迫初期可通过保持相对较高的APX、DHAR、GR活性, 促进AsA和GSH的再生, 但由于不结球白菜非耐涝植物, 后期仍导致植物根系中抗氧化物质含量降低, 严重影响了AsA-GSH循环的运作效率, 使过量产生的H₂O₂未能能够被及时清除, 进而影响植株生长。

关键词：不结球白菜; 淹水胁迫; H₂O₂; 抗坏血酸-谷胱甘肽循环

收稿：2015-07-22   修定：2015-11-17

资助：上海市绿叶蔬菜产业体系项目和“863”项目(2013AA-103006)。

Effects of Waterlogging Stress on Ascorbate-Glutathione Cycle in Brassica campestris ssp. chinensis

GUO Xin-Xin^1,2, LI Xiao-Feng¹, ZHU Hong-Fang¹, ZHU Yu-Ying^1,*, LIU Jin-Ping^1,2

¹Horticultural Research Institute, Shanghai Academy of Agricultural Science, Shanghai Key Lab of Protected Horticultural Technology, Shanghai 201106, China; ²College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: ZHU Yu-Ying; E-mail: yy5@saas.sh.cn; Tel: 021-52235455

Abstract:

The change rule of active oxygen content and ascorbate-glutathione cycle was analyzed under different treatment time (1, 3, 5, recovery 7 d) and different level (root flooded and half submerged) in non-heading Chinese cabbage (Brassica campestris ssp. chinensis). Waterlogging stress significantly increased the hydrogen peroxid content (H₂O₂) and the producing rate of superoxide anion radical (O₂ ¯• ) in Pak-choi seedlings. With the rising stress levels, the hydrogen peroxid content (H₂O₂) and the producing rate of superoxide anion radical (O₂ ¯• ) were increased, and they were still lower than the control after the relief of waterlogging stress. During the period of waterlogging stress, the abscisic acid (AsA) content, glutathione (GSH) content, the AsA/DHA value and GSH/GSSG value were significant reduced, while the dehydroascorbic acid (DHA) content and oxidized glutathione (GSSG) content were significantly increased. Early the stress, non-heading Chinese cabbage seedlings can maintain the relatively higher ascorbic acid peroxidase (APX), dehydroascorbic acid reductase (DHAR), glutathione reductase (GR) activity for resisting the stress and promote the synthesis of AsA and GSH. But the antioxidant substance content were reduced in the late and the AsA-GSH circulation efficiency were serious effected after the waterlogging stress, resulting in the produced excess H₂O₂ can not be cleared in time and plant growth were effected.

Key words: Brassica campestris ssp. chinensis; waterlogging stress; H₂O₂; ascorbate-glutathione cycle